Silva and Chilwell will make their Blues debuts against the Championship side at Stamford Bridge, but Hakim Ziyech will have to wait for his as he continues to recover from a knee injury.
Christian Pulisic is also not quite fit and will not be involved in the contest.
‘Chilwell in the squad for tomorrow,’ Lampard told a press conference on Tuesday afternoon. ‘Pulisic and Ziyech are getting closer.
‘He [Silva] is in the squad tomorrow. He is getting fit and will make a decision on how many minutes he gets.’
Neither is likely to play a full 90 minutes on Wednesday, but they will get some game time under their belts against the Tykes, who beat Nottingham Forest and Middlesborough to reach this stage, but have lost both their first two Championship games this season.
Chelsea are likely to have added Edouard Mendy to their ranks by the time they take on Barnsley, with Lampard confirming the goalkeeper was undergoing a medical at the club on Tuesday afternoon.
The 28-year-old will complete his move from Rennes imminently, although is unlikely to play on Wednesday night.