Having been hauled off at half-time against Everton in the opening Premier League match of the season, Alli was subsequently left out of games against Lokomotiv Plovdiv and Southampton entirely.
He played an hour in Macedonia on Thursday but was hooked with Spurs needing to find a winner, and he has now found himself left out of the matchday squad once again.
Tottenham have a gruelling fixture list at present, playing almost every two days, and while Mourinho admits rotation played a part in his thinking for the squad, he also says each player needs to ‘earn’ their place in his team.
Asked if the need to rotate accounted for Alli’s absence, or if there was another reason, Mourinho told Sky Sports: ‘A bit of both. We start with Harry Kane, with Son, with Lucas, and then on the bench we have Lamela and Bergwijn.
‘A little bit of rotation. And when the players are on the pitch they have to try to earn more minutes for the next match and for the next match they have to fight for that.’
‘You are comparing our team with the team that played against Southampton, but we had a match two days ago for Europa League and we have another one after tomorrow.
‘We need to change players, it’s impossible for the players to play every match. Tanguy played very well for 90 minutes – the first 90 minutes he did for quite a while – and of course today he cannot do it.’
Mourinho is believed to have an increasingly tense relationship with Alli, who he repeatedly branded ‘f***ing lazy’ in the Amazon All or Nothing documentary, and there have been suggestions he could be offloaded this summer with PSG interested.